Chemiluminescence

Chemiluminescence Professor Stewart CHM 151L-003 Group Members Melissa Spegal Jessica Buddi March 19, 2013 Megan Cochran Professor Stewart Chemiluminescence March 19, 2013 mental hospital The objective of this lab was to carry bring out a systematic pitch of look intos in exhibition to determine which combination of chemicals produce the brigh quiz and broadest indestructible legerity, through chemiluminescence. Chemiluminescence poop be defined as the emission of high spirits by a chemical reaction that does not produce heat.Chemiluminescence can also be found in nature, where it is referred to as bioluminescence. This can lots be found in umpteen compact sea fish, algae, and fireflies. Luminol is wizard of many chemicals that can be used to create chemiluminescence outside of nature. Scientists ar very interested chemiluminescence because it could be very helpful in many real life situations such as in impudencesticks. The military uses top-grade luminescencestick s to have light in field situations where electricity is not an option.Marine biologists and deep sea divers also use those glint sticks to provide light and make new discoveries in the deeps of the ocean or underwater caves. During the chemiluminescence experiment the goal was to produce light during a series of essays in order to create light or a glow. In order to do this systematically, all chemicals were used to start and as it became unpatterned that nearly chemicals did not effect the outcome of certain trials they were eliminated champion at a time.Because the trials were make systematically from one to the next, only one element of each trial was changed at a time,making it easy to single out the defining factors. at that place were many chemicals used during this experiment including Luminol, bleach, DMSO, NaOH, HCl, and H2O2. On the unsweetond mean solar sidereal day of trials, the Luminol was chilled to test the effects this would have on the glow we obtained through chemical reactions. Results of mean solar day 1 hedge 1Trial Luminol (D) Bleach (D) DMSO (D) 1M NaOH (D) 1M HCl H2O2 (D) Results A1 refinement 5* 1 5 2 5 3 5 4 5 5 Orange color A2 ghost 10 2 5 1 10 4 10 3 10 5 Brown A3 TAD 5 2 5 1 FLASH yellow A4 TAD 3 1 2 2 Slight blink of an eye A5 TAD 5 3 5 2 5 1 * Flash A6 TAD 5 3 5 2 10 1 Less twinkling B1 TAD 5 4 5 3 5 2 5 1 Flash B2 TAD 5 4 5 2 5 3 5 1 Flash B3 TAD 5 4 5 3 5 2 5 1 B4 TAD 5 4 5 3 10 2 5 1 Small flash B5 TAD 5 4 10 3 5 2 5 1 Blue flash B6 TAD 5 4 10 2 5 1 5 3 2. 7 sec flash C1 TAD 5 3 11 2 5 1 5 4 Small blasphemous flash after bleach C2 TAD 3 4 10 1 5 2 5 3 3. 2 sec flash C3 TAD 5 4 10 1 5 2 5 3 Small dingy flash C4 TAD 3 4 5 1 10 2 5 3 4 fosters flash/glow C5 TAD 10 4 2 1 10 2 5 3 6 sec glow C6 TAD 10 4 10 1 10 2 10 3 24. sec glow D1 TAD 10 4 10 2 10 1 10 3 D2 TAD 10 4 3 1 10 2 5 3 Small flash D3 TAD 5 3 5 2 5 1 Small flash D4 TAD 1 4 10 1 5 2 1 3 Flash D5 TAD 2 1 10 2 10 3 D6 TAD 4 1 10 2 10 3 , Asterick ( * ) stirred Highlighted golf-club of emplacement into the cell wells. The tad of Luminol was the first in every trial. (D) Drops go Highlighted course of study Best Trial of the Day Results of Day 2 Table 2Trial Luminol (mL) Bleach (D) DMSO (D) H2O2 (D) NaOH Results A1 TAD 10 4 10 1 10 3 10 2 Quick glow odor A2 1 1 5 2 5 3 5 2 glow A3 1 1 5 4 5 2 5 3 glow A4 1 1 5 3 10 2 glow A5 1 3 5 4 5 2 10 1 glow A6 1 1 5 3 5 2 glow B1 1 1 5 3 5 2 * long glow B2 1 1 5 4 5 2 5 3 * B3 1 1 5 3 5 2 * 36 sec glow B4 1 1 10 3 10 2 * 23 flashs B5 1 1 5 3 10 2 * Bright but shorter B6 1 1 5 3 5 2 * C1 1 1 5 4 10 2 2 3 * C2 1 1 5 3 20 2 * 29 Secs C3 1 3 1 2 1 1 * Chilled *Stirred Highligh ted Order of placement into the cell wells. The tad of Luminol was the first in every trial. Drops Pink Highlighted Row Best Trial of the Day Table 3 MSDS Chemical figure Ingestion Skin Contact Disposal Inhalation Luminol Loosen clothing, if not breathing per fake mouth to mouth recessitation. Do not defecate vomit. Wash with lots of water. Cover skin with emollient. Not Available Rest. aerate Area, seek medical attention. Bleach Drink Water. Do not father vomiting. Wash skin with water for 15-20 minutes. Containerize and use absorbants on liquid. Remove, honeyed port. DMSO Loosen clothing, if not breathing perform mouth to mouth recessitation. Do not induce vomit. Wash with soap and water. Waste container. Fresh air Discussion The take up trial of the experiement was on day two, trial B3. During that trial 1 mL cold Luminol was used and added to the cell well first, followed by flipper drops of DMSO, and five drops of bleach in that order. This was the best trial because it yielded the intenseest and long-acting lasting glow compared to all of the others. On the trial before the said(prenominal) exact method was carried out except there was hydrogen hydrogen peroxide in the mix also. In order to change things up, the peroxide was eliminated and that proved to be an effective tactic.It became blatently apparent that some of the chemicals were not needed solely including the NaOH, and HCL. By the trial B6, on the second da,y the HCL had been eliminated. Hydrogen peroxide was never eliminated but it was noted as in trial B3 on the second day that the longest trial was performed without it. Many factors affected the results of each trial, some definitely more than others. For instance, for the entire first day, all trials were performed with the solid form of Luminol, and on the second day, starting with trial A2, the Luminol stock solution was used. As reflected in the table above, the stock solution created a oftentimes long -lasting glow on average.To furthur amplify the Luminols effect from trial B1 on day two, the Luminol stock solution was used in a chilled form which created the best results of both days as seen in trial B3. It also became apparent that stirring the chemicals helped maintain the glow longer per trial C2 on day two. Luminol was the only chemical that was chilled, all other chemicals remained room temperature. The order of chemicals was an primary(prenominal) factor in creating chemiluminescence as well. During the beginning trials of the first day the best order to add the chemicals was not apparent, but by trial C4 one thing was certain, the luminol needed to be the first chemical placed in the well. In trial C3, when Luminol was added last, there was only a small blue flash with no lingering glow at all.Also, by the second day, it was realized that the bleach was reacting with the Luminol and if the bleach was added last, the glow did not fizzle out as quickly such as in trial B4 on the second day, which had a 23 second glow, subsequently improving results on day two as opposed to the first day. After completeing much more in-depth research on the topic of chemiluminescence other checmicals had been foun, that if the experiment was do over would have been requested, such as copper nitrate, which would have importantly extended the length of the glow. Conclusion Using 1mL of chilled Luminol stock solution, five drops of DMSO, and five drops of bleach, in that order and stirring at the end, the longest glow of 36 seconds was created as per trial B3 on day two. The bleach reacting with the Luminol gave a bright glow, and the DMSO aided in the length of time the trial glowed.